How to reconstitute Semaglutide
Semaglutide is sold as a freeze-dried powder, most often in 2 mg, 3 mg, 5 mg, 10 mg vials. Add bacteriostatic water, let it dissolve without shaking, and the concentration is the vial amount divided by the water: 5 mg in 2 mL is 2.5 mg/mL. On a U-100 insulin syringe one unit is 0.01 mL, so each unit carries 25 mcg, and a 250 mcg dose is 10 units.
The Wegovy label starts at 0.25 mg once weekly for four weeks and steps through 0.5, 1, 1.7 and 2.4 mg; Ozempic tops out at 2 mg. Nothing on this page is a dosing recommendation; the calculator converts a dose you already have into syringe units.
Semaglutide doses in syringe units (5 mg vial, 2 mL water)
| Dose | Units | mL | Doses per vial |
|---|---|---|---|
| 250 mcg | 10 | 0.1 | 20 |
| 500 mcg | 20 | 0.2 | 10 |
| 1 mg | 40 | 0.4 | 5 |
| 1.7 mg | 68 | 0.68 | 2 |
| 2.4 mg | 96 | 0.96 | 2 |
Vial sizes at 250 mcg per dose
| Vial | Water | Concentration | Units per dose | Doses |
|---|---|---|---|---|
| 2 mg | 2 mL | 1 mg/mL | 25 | 8 |
| 3 mg | 2 mL | 1.5 mg/mL | 16.7 | 12 |
| 5 mg | 2 mL | 2.5 mg/mL | 10 | 20 |
| 10 mg | 2 mL | 5 mg/mL | 5 | 40 |
The dose column is in mg. Doubling the water halves the concentration and doubles the units per dose; the number of doses in the vial never changes. If a dose comes out under 5 units, use less water so it is easier to measure. If it exceeds the syringe, use more water or a bigger syringe.
Worked example
5 mg / 2 mL = 2.5 mg/mL = 2,500 mcg/mL. One unit = 0.01 mL = 25 mcg. 250 mcg / 25 mcg per unit = 10 units. The vial holds 5,000 / 250 = 20 doses, which at 1 per week is about 140 days.
See the general peptide calculator for the formulas, syringe sizes, and the common mg versus mcg mistakes.